Plasmid_Backbone

Part:BBa_M36094:Design

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)


S. elongatus cyanobacteria actuator test rig


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2580
    Illegal PstI site found at 315
    Illegal PstI site found at 2190
    Illegal PstI site found at 4754
    Illegal PstI site found at 5994
    Illegal PstI site found at 6327
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2580
    Illegal PstI site found at 315
    Illegal PstI site found at 2190
    Illegal PstI site found at 4754
    Illegal PstI site found at 5994
    Illegal PstI site found at 6327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 4181
    Illegal BamHI site found at 4191
    Illegal BamHI site found at 6736
    Illegal XhoI site found at 4910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2580
    Illegal PstI site found at 315
    Illegal PstI site found at 2190
    Illegal PstI site found at 4754
    Illegal PstI site found at 5994
    Illegal PstI site found at 6327
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2580
    Illegal PstI site found at 315
    Illegal PstI site found at 2190
    Illegal PstI site found at 4754
    Illegal PstI site found at 5994
    Illegal PstI site found at 6327
    Illegal NgoMIV site found at 6762
    Illegal NgoMIV site found at 7134
    Illegal NgoMIV site found at 7294
    Illegal NgoMIV site found at 7648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 141
    Illegal BsaI.rc site found at 4452
    Illegal SapI site found at 8715


Design Notes

Source plasmid is pNS2-YFP. pNS2-YFP is a plasmid made by Rachel Hood from Dr. Dave Savage's lab.


Source

Redesigned by the BIOE44 teaching staff from pNS2-YFP.

References